Antioxidant effect of hemidesmus indicus on ethanol induced hepatotoxicity in rats. Rosmarinic acid attenuates the hepatotoxicity induced by. Hereby, we analyzed the toxic effects of malathion on the histological structure of liver and biochemical parameters in male rats. Antioxidant effect of hemidesmus indicus on ethanolinduced hepatotoxicity in rats article pdf available in journal of medicinal food 104. Same 25 mgkgintramuscularly three times a day was administered forfive consecutive days, while dlpc was administeredintraperitoneally for five days. Alcoholinduced increases in cytochrome p450 2e1 cyp2e1, which metabolizes alcohol and other molecules and generates ros in the process. Apap hepatotoxicity is caused by its reactive metabolite nacetylpbenzoquinone imine napqi, which causes oxidative stress and glutathione gsh depletion, a prerequisite for apap induced hepatotoxicity 6, 7. The presence of flavonoids, tannins, saponins and terpenoids incoccinia grandis explain its role in hepatoprotection by inhibiting the free radicals mediated. Ethanol treatment to albino rats for 8 weeks induced a fair degree of derangement of liver structure and function. Dili has become the leading cause of acute liver failure and transplantation in western countries 1, 2. Study of acute hepatotoxicity of equisetum arvense l. Alcoholinduced increases in the levels of free iron which can promote ros generation. Intrinsic hepatotoxicity following acetaminophen apap.
Hepatotoxicity was induced by single dose of acetaminophen apap. In the analysis of the hepatotoxicity of drugs found in livertox, fewer drugs than expected had documented hepatotoxicity. Pdf twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided into two groups of 6 rats each. Acute and chronic hepatotoxicity and nephrotoxicity study of. This can be very serious and can further lead to liver failure. Hepatoprotective effect of silymarin silybum marianum on. Evaluation of hepatoprotective and antioxidant activity of ichnocarpus frutescens linn. Drug induced hepatotoxicity is the most common reason cited for withdrawal of already approved drugs from the market and accounts for more than 50 percent.
Evaluation of hepatoprotective and antioxidant activity of. Administering 20% ethanol 5 gkg of body weightday for 60. Rats fed ethanol from 21 to days were subjected to one or more episodes of hypoxia. The hydroethanolic extract of hybanthus enneaspermus and silymarin treatment decreases the levels sgot,sgpt, alp and total bilirubin when compared to paracetamol treated rats. Phorate induced hepatotoxicity in rats sangeetha g s, muraleedhara kurup, helen a dept. Albino rats abstract toxic effects of gasoline fumes have been reported, but evidence of its hepatotoxicity and genotoxicity are rare. Ethanolinduced free radicals and hepatic dna strand breaks are prevented in vivo by antioxidants. Neither oral nor intraperitoneal treatment with acetaldehyde had any hepatotoxic effect, even following aldehyde dehydrogenase inhibition by. Hepatotoxicity can occur for different reasons and is liver damage that results in your liver not functioning properly. While methotrexate treated rats of group iii and iv showed weight loss on seventh day onward, there was no significant p free access to water and pellet diet. The rats of group i received only deionised water as control while, group ii, iii and iv were given lead. Druginduced liver injury dili therefore poses a major clinical problem.
An experiment was conducted to study the haematobiochemical alterations induced by lead acetate toxicity in 48 wistar rats of either sex, divided uniformly into four different groups. Six hours after this treatment, the rats were injected with acetaminophen 0. The rats were divided into eight groups of which two served as control group i. Three groups received daily different amounts of malathion. Nacetyl cysteine, hepatotoxicity, carbon tetrachloride carbon tetrachloride is commonly used as a model to evaluate hepatotoxicity 1. A single oral dose of 20 or 50 mgkg ccnu in female wistar rats induced an important increase in transaminases between day 2 and day 6, followed by a second, moderate increase between day 21 and day 28. Jul 11, 2015 the increasing use of organophosphorus pesticides in the environment constitutes an ecotoxicological hazard especially for humans and nontarget animals. The aim of the present study was to investigate the hepatoprotective activity of oleogumresin ogr in rats. Drug induced liver injury dili therefore poses a major clinical problem.
Animals of groups 3, 4 and 5 were treated with oc 100, 250 and 500 mgkg, po for 4 days and. Hepatoprotective effect of carob against acute ethanolinduced. Liver damage was induced in rats by administering ethanol 5 gkg, 20% wv p. Starvation significantly decreased the urinary excretion of both cs2 and its bivalent sulphur. A variety of mechanisms have been suggested to play important roles in pathways of ethanol toxicity to the liver. Atorvastatin attenuates ethanolinduced hepatotoxicity via. Ebong department of biochemistry, college of medical sciences, university of calabar, calabar, nigeria email. Ethanol cytotoxicity to a transfected hepg2 cell line. Hepatotoxicity was induced in rats by oral administration of. Abstract the present study was carried out to determine the severe hepatotoxicity and nephrotoxicity of gasoline on liver, kidney, hematological and oxidative stress parameters in wistar male albino rats. Numerous plant resin content essential oils with terpenes and sesquiterpenes have shown hepatoprotective activity.
Can phytochemicals be effective in preventing ethanol. Antioxidant effect of livomap, a polyherbal formulation on. Administering ellagic acid to rats with ethanol induced hepatotoxicity was shown to ameliorate the increased serum levels of aminotransferases, lipid peroxides, and hydroperoxides and also to reduce the elevated hepatic contents of cholesterol, free fatty acids, triglycerides, and phospholipids. Effect of oleogumresin on ethanolinduced hepatotoxicity in.
Subacute toxicopathological studies of methotrexate in wistar. Amelioration of antihepatotoxic effect by lichen rangiferinus against. We found that the optimal dose for alf induction in rats is 350 mg. The ability of acetaldehyde to initiate hepatotoxicity as evidenced by enzyme leakage, hepatic fat accumulation and histological alterations was studied in rats.
Hepatocyte oxygen and ethanol metabolism is elevated, resulting in hypoxia in downstream regions of the liver. The hydroethanolic extract of hybanthus enneaspermus was investigated for its hepatoprotective effect. Hepatotoxicity of acetaldehyde in rats sciencedirect. Paracetamol induced hepatotoxicity12 rats were divided into following six groups of 6 each. Effect of oleogumresin on ethanol induced hepatotoxicity in rats. The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanolinduced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats. Paracetamolinduced hepatotoxicity12 rats were divided into following six groups of 6 each. To evaluate the acute hepatotoxicity of equisentum arvense l. Alcohol, oxidative stress, and free radical damage. Hepatotoxicity and genotoxicity of gasoline fumes in albino rats. Increased hepatotoxicity of acetaminophen after chronic. Inhalation exposure also made it possible to study the effect of starvation on liver damage in phenobarbitonepretreated rats exposed to cs2. The crude methanol extract of the whole plant of f.
Prior a sohelia korourian e emanuele albano f magnus ingelmansundberg c dennis r. To evaluate the effect of acute ethanol administration on the hepatoxicity of acetaminophen, young male spraguedawley rats b. May 01, 2011 the hydroethanolic extract of hybanthus enneaspermus and silymarin treatment decreases the levels sgot,sgpt, alp and total bilirubin when compared to paracetamol treated rats. Effect of naringenin on cadmiuminduced changes in the levels of ast and alt in liver of control and experimental rats. Current concepts of mechanisms in druginduced hepatotoxicity. Pdf cadmium induced hepatotoxicity in rats and the. Some rats and hepatocytes were treated by resveratrol or. Hepatoprotective effect of flaxseed and its protein on ethanol. Hepatoprotective effect of hydroethanolic extract of. B 250 mgkg and 500 mgkg produced p in paracetamol induced liver marker enzymes and total bilirubin, but its 750 mgkg showed remarkable decline in alt, ast, alp and tb levels, compared to the reference. Ra was given one hour prior to etoh by oral gavage for 28 days. Sirtuin 1 modulation in rat model of acetaminopheninduced.
The experimental animals were randomized into four groups n 6 which ran simultane. The increasing use of organophosphorus pesticides in the environment constitutes an ecotoxicological hazard especially for humans and nontarget animals. There is currently no way to reimburse for the absence of liver function. Jambilingappa, evaluation of hepatoprotective activity of aqueous extract of azadirachta indica neem leaves against paracetamol induced hepatotoxicity in albino rats, pharmacologyonline, vol.
The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanol induced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats. The hepatoprotective activity of conyza bonariensis ethanol extract was studied against paracetamol induced hepatotoxicity in mice. Effects of nacetylcysteine on ethanolinduced hepatotoxicity. Effects of nacetylcysteine on ethanol induced hepatotoxicity in rats fed via total enteral nutrition author links open overlay panel martin j. Protection of hepatotoxicity using spondias pinnata by. Hypervitaminosis a enhances hepatotoxicity of ethanol in rats hypervitaminosis a enhances hepatotoxicity of ethanol in rats 19830701 00. Article information, pdf download for hepatoprotective effect of carob against.
The present study was carried out to determine the severe hepatotoxicity and nephrotoxicity of gasoline on liver, kidney, hematological and oxidative stress parameters in wistar male albino rats. Acetaminopheninduced hepatotoxicity in the presence of ethanol has not been studied. The effect of ethanol extract mymercodia pendans on paracetamol. Effect of oleogumresin on ethanolinduced hepatotoxicity in rats. Previous studies showed statins reduce lipid peroxidation and attenuate free radical damage and exhibit antioxidant activity against hydroxyl and proxy radicals. Apap hepatotoxicity is caused by its reactive metabolite nacetylpbenzoquinone imine napqi, which causes oxidative stress and glutathione gsh depletion, a prerequisite for apapinduced hepatotoxicity 6, 7. Cyp2e1, oxidative stress and mapk signaling pathways in. Acute and chronic hepatotoxicity and nephrotoxicity study of orally administered chloroform extract of carica papaya seeds in adult wistar rats 1umana, uduak e. Some of these include effects of ethanol on mitochondrial functions and bioenergetics, membrane fluidity, and altered signal transduction, redox state changes as a consequence of ethanol oxidation, effects on the immune system or on eicosanoid metabolism. Antioxidant effect of livomap, a polyherbal formulation on ethanol induced hepatotoxicity in albino wistar rats kanchana. Therefore, this study assesses hepatotoxicity and genotoxicity of gasoline fumes on forty albino rats randomly assigned to. Biochemical markers for liver injury and hematological indices of all animals were.
Organophosphorus compounds induce oxidative stress leading to generation of free radicals and alterations in antioxidant and scavengers of oxygen free radicals. They had free access to a commercial balanced stock diet and water. In this study, the rate of tramadol metabolism in rats with damaged liver induced by ethanol and acetaminophen was assessed in a recirculation perfusion system. Implication of free radical mechanisms in ethanolinduced cellular injury. Pdf antioxidant effect of hemidesmus indicus on ethanol. Protective effect of genistein against nnitrosodiethylamine. Fortyeight 48 male wistar albino ratswith weight range of 187245g divided into four groups with two replicates were allowed to acclimatize to laboratory condition for 14 days. Preliminary toxicity study to determine the volume of gasoline that could cause toxicity was carried out using 56 healthy male albino rats. Acetaminophen induced hepatotoxicity in the presence of ethanol has not been studied. Group 1 normal control group and group 2 toxin control group received 0.
Haematobiochemical alterations induced by lead acetate. Prevention of acetaminopheninduced hepatotoxicity by acute. Alkaline phosphatases and conjugated hyperbilirubinemia threefold. Pdf the effect of ethanol extract mymercodia pendans on. Nnitrosodiethylamine ndea induced hepatotoxicity in swiss albino rats fahad ali, rahul, falaq naz, smita jyoti, yasir hasan siddiquen section of genetics, department of zoology, faculty of life sciences, aligarh muslim university, aligarh, uttar pradesh 202002, india received february 2014. Among 671 drugs available for analysis, 353 53% had published convincing case reports of hepatotoxicity. Dominant lethal mutations have been induced by oral exposure of spraguedawley rats klassen and persaud, 1978, longevans rats mankes et al. Fagonia olivieri dc being used for the treatment of diabetes, cancer, fever and claimed to be effective in many other stress related disorders. This is mainly due to the effective antioxidant potential of nacetyl cysteine. Hepatotoxicity of acetaminophen after chronic ethanol feeding for 46 wk, serum got activity was increased significantly compared with pairfed controls 81. Severe hepatotoxicity and nephrotoxicity of gasoline petrol.
Some rats and hepatocytes were treated by resveratrol or synthetic selective activator of sirtuin 1 cay10591. In overdoses, it is a potent hepatotoxin, producing fulminant hepatic and renal tubular necrosis, which can be lethal in human and. E ect of quercetin on paracetamolinduced rat liver. Its major influence is on the important cause of liver injury. The effect of ethanol extract mymercodia pendans on paracetamol induced hepatotoxicity in white rats article pdf available in iop conference series earth and environmental science 248. Amelioration of paracetamolinduced hepatotoxicity in rat by. Material and methods male wistar rats weighing 200350 g were used. Hepatoprotective activity of sphaeranthus amaranthoides whole plant in paracetamol induced hepatotoxicity in rats. Pdf ethanol induced hepatotoxicity in albino rats researchgate. Second, we examined whether wistar and lewis rats exhibit any differences in the course of alf induced by different taa doses.
Original article antioxidant and hepatoprotective effect of. A few cases of liver involvement have been reported in patients receiving treatment with the antineoplastic nitrosourea ccnu. Phorate induced hepatotoxicity in rats iosr journals. Neither oral nor intraperitoneal treatment with acetaldehyde had any hepatotoxic effect, even following aldehyde dehydrogenase inhibition by disulfiram. Malathioninduced hepatotoxicity in male wistar rats.
Wistar rats proved more susceptible to the development of taainduced alf compared with lewis rats. Drug induced hepatotoxicity is the most common reason cited for withdrawal of already approved drugs from the market and accounts for more than 50 percent of cases of acute liver failure in the united states. Various mechanisms may be associated with the liver damage. Fifty wistar rats were used, these being divided in four groups, one being the control receiving only water and the other groups receiving graded doses of equisentum arvense l. Prevention of ethanolinduced liver injury in rats by an. Male wistar rats were used for both in vivo and in vitro studies. Wistar rats proved more susceptible to the development of taa induced alf compared with lewis rats. Acetaminophen is a mild analgesic and antipyretic agent, which can cause centrilobular hepatic necrosis in toxic doses, whereas alcohol causes death due to liver diseases. Hepatoprotective activity of ethanol extract of conyza b. Effects of nacetylcysteine on ethanolinduced hepatotoxicity in rats fed via total enteral nutrition author links open overlay panel martin j. Hypervitaminosis a enhances hepatotoxicity of ethanol in rats.
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